An Easy Method for Determinaion of Dioxins:the CALUX Assay
- VernacularTitle:二噁英类污染物的CALUX测定法
- Author:
Ying LI
;
Yihe JIN
- Publication Type:Journal Article
- Keywords:
Laboratory techniques and procedure;
Chromatography,gas;
Environmental pollutants;
Dioxin
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
This article introduces a new method: the chemical-activated luciferase gene expression (CALUX)assay for determine of dioxin,which is based on biological properly of a genetically modified cell line.The published thesis reports that the contents of dioxins in a same sample abtained from the CALUX assay and the internationally recoganized standard method for determination of dioxin: high resolution gas chromatography-mass spectrometry(HRGC-MS)showed perfectly positive correlation and well repeatibility. The CALUX assay presents less amount-required sample,shorter determination cycle and less expense compared with HRGC-MS assay. The CALUX assay can be used as a rapid screen assay for determination of dioxin in a large number of samples,and will be widely applied in the survey of dioxin pollution in environment.
