Speciation Analysis of Selenium in Plant Samples by Microwave-Assisted Digestion-Hydride Generation Atomic Fluorescence Spectrophotometry
- VernacularTitle:植物中硒的分离和微波消解鄄原子荧光测定法
- Author:
Lingjin ZHANG
;
Dexun CHEN
;
Xiaoduan LIU
- Publication Type:Journal Article
- Keywords:
Spectrophotometry;
Selenium;
Microwave-assisted digestion
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for speciation analysis of selenium in plant samples by H2O2-HNO3 microwave-assisted digestion-HGAFS. Methods Plant samples were prepared using H2O2-HNO3 by microwave-assisted digestion. The contents of soluble inorganic Se(IV)and Se(VI)in the samples were determined by HGAFS after separation most of the soluble organic compounds by XAD-2 resin column in HNO3 medium at pH=3. The contents of soluble Se,insoluble Se,inorganic Se (IV)and Se (VI)and organic Se could be calculated. Results The linear range and detection limit of Se(IV)were 0-80 ?g/L and 0.05 ?g/L respectively. The RSD for the total Se detection was 1.7%(n=5?雪. The total Se content in the reference standard substance obtained by this method agreed well with the reference value. Conclusion The method for preparation of plant samples using microwave-assisted digestion could avoid the loss of Se through volatilization. The pretreatment using XAD-2 resin could remove the interference of organic compounds on detecting results of Se(Ⅳ)in the plant samples,which could widen the application of the method in the speciation analysis. The analytical scheme of the method could determine the contents of total,soluble,insoluble,soluble inorganic Se(IV?雪?Se(VI?雪,and organic Se in plant samples. The method was simple and practical.
