Amplification of SAG3 gene fragment and its application in detection of toxoplasma gondii in hemato- poietic stem cell transplantation
- VernacularTitle:SAG3基因在造血干细胞移植患者弓形虫感染诊断中的应用
- Author:
Yongan ZHOU
;
Xinbing YU
;
Zhongdao WU
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell transplantation;
Toxoplasmosis;
Polymerase chain reaction;
Immunoassay;
Genes
- From:
Chinese Journal of Organ Transplantation
1996;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid, specific and sensitive diagnostic technique for the human T. gondii infection with hematopoietic stem cell transplantation and discuss its clinical significance. Me- thods Fifty-six patients subject to hematopoietic stem cell transplantation were detected with ELISA and PCR. Results Among 56 recipients of hematopoietic stem cell transplantation, 7 were positive for T. gondii antigen and 10 were positive for SAG3 gene fragment respectively with the positive rate being 14.3 % and 17.8 % in the ELISA and PCR screening respectively. Twenty healthy people were negative for anti-Toxo antibody.Conclusion PCR is an accurate, relatively rapid, sensitive and specific method for detecting SAG3 gene of T. gondii, and can be considered a valuable additional tool for identification of T. gondii infections.
