Marked prolongation of murine cardiac allograft survival using recipient immature dendritic cells pulsed donor-derived apoptotic cells
- VernacularTitle:负载供者凋亡细胞的受者未成熟树突状细胞可延长小鼠移植心脏的存活时间
- Author:
Dongliang XU
;
Xiaoda TANG
;
Jianming TAN
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Dendritic cells;
Mice;
Heart transplantation
- From:
Chinese Journal of Organ Transplantation
1996;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of recipient dendritic cells (DC) loaded with donor-derived apoptotic cells on inducing murine cardiac allograft tolerance. Methods Apoptosis of donor-derived splenocytes (SC) was induced by ultraviolet B irradiation(UVB). UVB-irradiated allogenic SC were co-cultured with recipient bone marrow-derived DC that maturation was inhibited by NF-?B ODN Decoy, so that could acquire tolerogenic-immature DC loaded with donor-derived apoptotic cells (Decoy Apo-SC DC). A heterotopic vascularized heart transplantation was performed from BALB/c to C57BL/6 mice, and recipients were given one injection of recipient immature (Decoy Apo-SC DC) or mature (Apo-SC DC) DC engulfed donor-derived apoptotic cells (2?10 6 cells) through the portal vein at 7 days before the heart transplantation in the absence of immunosuppression. The cardiac survival and the expression of intragraft cytokines (IL-2, IL-10 and IFN-?) were evaluated.Results DC had potent phagocytosis of allogenic apoptotic SC (Apo-SC). NF-?B ODN Decoy inhibited engulfment of apoptotic cells-induced maturation of DC and then induced recipient tolerogenic DC. Recipient tolerogenic DC loaded with donor-derived apoptotic cells were able to cross-tolerate recipient T cells, which revealed by alloantigen-specific T-cell hyporesponsiveness in primary and secondary mixed leukocyte reaction. Injection of recipient tolerogenic DC loaded with Decoy Apo-SC DC through the portal vein at 7 days before the heart transplantation significantly prolonged vascularized heart allograft survival (MST 36.4 days versus 7 days in control group, P
