Immune active effects of chemokine RANTES on human peripheral mononuclear cells
- VernacularTitle:趋化因子RANTES对人外周血单个核细胞的免疫活化作用
- Author:
Xiao GU
;
Xiaoda TANG
;
Shenyang GU
- Publication Type:Journal Article
- Keywords:
RANTES;
Signal transduction;
Immunization;
Immunocompetence
- From:
Chinese Journal of Organ Transplantation
1996;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferation and phenotypes of peripheral mononuclear cells (PMNC) stimulated by recombinant human RANTES (rhRANTES) and the mechanisms involved.Methods PMNC was stimulated by various concentrations of rhRANTES and/or anti-CD3 mAb and intervened by PDTC and CTLA4Ig. Scintillation counter was used to count the cpm of proliferation cells and flow cytometry was used to detect the phenotypes of lymphocytes.Results rhRANTES was capable of directly stimulating purified human PMNC proliferation and two peaks occurred with rhRANTES concentration of 100 ng/ml and 5000 ng/ml respectively. The proliferation of PMNC stimulated by rhRNATES was significantly higher than that in the presence of anti-CD3mAb (P
