The role of DR5 in TRAIL-induced apoptosis for Jurkat cell line
- VernacularTitle:DR5在TRAIL诱导Jurkat细胞凋亡中的作用
- Author:
Yuanfang MA
;
Dongliang YANG
;
Shixin LU
;
Youhai CHEN
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-related apoptosis inducing ligand;
Death receptor 5;
Apoptosis;
Anti-DR5 mAb
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the role of DR5 in TRAIL apoptotic signal transduction. Methods:BALB/C mice were immunized with recombinant DR5 that contains the full-length extracellular domain of the human DR5. Anti-DR5 mAb was generated by hybridoma. The level of DR5 expression on Jurkat cell line was examined by flow cytometry. The rates of TRAIL-induced apoptosis and anti-DR5 mAb blocking on Jurkat cells were tested by flow cytometry with TRAIL apoptosis kit.Results:The percentage of DR5 expression on Jurkat cells was 94 83%. TRAIL and anti-TRAIL mAb could kill Jurkat cells on dose-dependent, and the killing rate was 90% in the concentration of 50～100 ng/ml. The killing role of TRAIL could be blocked on Jurkat cells pretreated with anti-DR5 mAb. The average percentage of blocking was 90 49%.Conclusion:DR5 plays a very key role in TRAIL induced apoptosis in Jurkat cells.
