Experimental study on relationship between ?_3-adrenoceptors and detrusor instability secondary to bladder outflow obstruction
- VernacularTitle:肾上腺素?_3受体与梗阻后逼尿肌不稳定关系的实验研究
- Author:
Wenfeng CAO
;
Bo SONG
;
Xiyu JIN
- Publication Type:Journal Article
- Keywords:
Detrusor;
Obstruction;
Rats;
?3-adrenoceptors
- From:
Chinese Journal of Urology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ? 3-adrenoceptors(? 3-AR) on rat detrusor instability(DI) secondary to bladder outflow obstruction(BOO). Methods Animal models of DI were made in 15 female Wister rats.The filling cystometry was conducted after 6 weeks,and the rats were divided into DI group and detrusor stabitily (DS) group based on the detrusor function. The effects of ? 3-AR agonist BRL37344A on the frequency of contraction and relaxation response to isolated detrusor were examined by means of the in vitro detrusor strip study.And the expression of ? 3-AR subtypes mRNA was investigated in rat detrusor muscle by RT-PCR. Results The occurrence rate of DI was 67%(10/15).BRL37344A could inhibit the contraction frequence and amplitude of the isolated detrusor which was concentration-dependent.The intensity did differ significantly between normal controls or DS group and DI group.The relative contents of ? 3-adrenoceptor mRNA in DI group,controls and DS group were 10.27?3.54,19.84?2.62 and 18.38?1.95.There was a significant decrease in the concentration of ? 3-adrenoceptors in DI group compared with controls and DS group( P
