Preliminary study of rhBMP-2 induced bone ingrowth at the interface into hollow implants
- VernacularTitle:rhBMP-2诱导界面骨长入中空金属植入物的初步实验研究
- Author:
Xu CAI
;
Yan WANG
;
Jifang WANG
- Publication Type:Journal Article
- Keywords:
Prostheses and implants;
Bone morphogenetic proteins;
Growth
- From:
Chinese Journal of Orthopaedics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to explore the possibility of bone ingrowth into a hollow prosthesis at the bone-implant interface, and the promoting effects induced by rhBMP-2/carrier compound as well. Methods Bone harvest chamber (BHC) modified form Albrektsson designation, was implanted into meta-physis of tibia of New Zealand rabbit to establish an animal model. 10 ?l rhBMP-2 and rhBMP-2/Healon GV compound, each of which contained 1mg rhBMP-2, were loaded into BHC respectively. The same vol-ume of Healon GV and injection water was also loaded into as control. The tissues in the BHC were harvest- ed at 2, 4 and 6 weeks after implantation respectively. Histological observation with non-decalcified slicing technique was adopted, and bone histomorphometry was analyzed by computerized image analysis system to calculate TBV (trabecular bone volume). The data was compared among the different groups. Results At two weeks after loading, there were woven bone formation in groups of rhBMP-2/Healon GV compound and rhBMP-2, and mature bone trabeculae with marrow-like tissue were observed at 4 weeks; At 6 weeks, new bone formation in the BHC became more intensive. However, the tissues in BHC of Healon GV and injection water at 4 weeks were just like those of the former two groups at 2 weeks, and mature bone trabeculae with marrow-like tissue could only been found until 6 weeks. Conclusion Bone at the interface can grow into the contained space of hollow prosthesis through holes at the wall, and form mature bone. rhBMP-2 loaded in the hollow space can obviously promote this effect and the carrier Healon GV has no negative influence but can increase bone inductive effect.
