Effect of thyroid stimulating antibody on thyroid peroxidase activity in cultured thyrocytes
- VernacularTitle:甲状腺刺激抗体对甲状腺细胞过氧化物酶活性影响
- Author:
Yi ZHANG
;
Guoliang HUANG
;
Yu YANG
;
Jianrong LI
- Publication Type:Journal Article
- Keywords:
Thyroid stimulating antibody;
Thyroid peroxidase;
Signal transduction pathway;
Graves′ disease
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of thyroid stimulating antibody (TSAb) on the thyroid peroxidase (TPO) activity and its signal transduction pathways in cultured thyrocytes. Methods The TPO activity in primary cultured human thyrocytes was determined by amended guaiacol oxidation assay. At the same time, inhibitors of signal transduction were applied and the roles of cAMP/protein kinase A (PKA), phosphatidylinositol (PIP 2)/Ca 2+and tyrosine protein kinase (TPK) pathway on the TPO activity in cultured thyrocytes were observed. Results TSAb significantly enhanced the TPO activity in cultured thyrocytes, and the peak of TPO activity (360% of controls) reached with 4 mg/ml TSAb for 48 h. Addition of PKA inhibitor for blocking cAMP/PKA pathway decreased TPO activity by 79.2% (P
