In vitro effects of calcitonin on osteoclasts
- VernacularTitle:降钙素对体外培养破骨细胞功能的影响
- Author:
Xiuzhen ZHANG
;
Junfeng HAN
;
Guofeng QIAN
- Publication Type:Journal Article
- Keywords:
Calcitonin;
Osteoblast;
Osteoclast;
Cell culture
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of calcitonin on osteoclast activity and expressions of osteoprotegrin (OPG) and receptor activator of NF-?B ligand (RANKL) in vitro. Methods Osteoclasts and their precursors were mechanically isolated from long bones of SD rats and incubated on bone sheet in MEM with different concentration of calcitonin. Number, morphology of the osteoclasts were detected with tartrate-resistance acid phosphatase (TRAP) staining. Image-Pro Plus counted the resorption pits and areas on slices, stained by toludine blue. RT-PCR was used to examine the expressions of OPG and RANKL of osteoclast. Results Number of osteoclasts were decreased with increasing CT concentrations. After incubation for 5 days, resorption pits were decreased in a concentration-dependent manner (P