Effects of the exogenous PTEN tuomr suppressor gene on morphology of the highly metastatic mucoepidermoid carcinoma cell line M3SP2
- VernacularTitle:外源性PTEN抑癌基因对高转移性黏液表皮样癌细胞系形态的影响
- Author:
Bin LIU
;
Junzheng WU
;
Zhenqiang SITU
- Publication Type:Journal Article
- Keywords:
Mucoepidermoid carcinomap;
PTEN, Gene transfection, Morphology
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objeact:To evaluate the effects of the exogenous PTEN gene on morphology of the highly metastatic mucoepidermoid carcinoma cell line M3SP2. Methods: Morphological observation of vehicle transfected M3SP2-pBp cells and PTEN transfected M3SP2-PTEN cells was performed with inverted microscope, HE staining and optical microscope, scanning electronic microscope and transmisson electronic microscope. Results: Compared with the control cells of M3SP2-pBp, the exogenous PTEN expressing cells M3SP2-PTEN showed morphological changes, such as vacuole denaturalization, shrinkage, less microvillus, chondriosome swelling, lysosome amalgamation, and chromatin agglutination. Conclusion: The exogenous PTEN gene may induce denaturalization, necrosis, and apoptosis of the highly metastatic mucoepidermoid carcinoma cells.
