Development of nanometer hydroxyapatite complex materials——Cytotoxicity test of nano-hydroxyapatite by MTT-assay
- VernacularTitle:纳米羟基磷灰石复合材料的研究——纳米羟基磷灰石的细胞毒性
- Author:
Yanhui XU
;
Siqun LI
;
Zhian LI
- Publication Type:Journal Article
- Keywords:
Nano-hydroxyapatite;
Cytotoxicity;
MTT assay
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the cytotoxicity of nano-hydroxyapatite. Methods: Mouse lung fibroblast L-929 and primary cultured human periodontal ligament fibroblast-like cells (PDLF) were cultured in the medium of extraction of nano-hydroxyapatite or commerce hydroxyapatite at 100%,50%,10% and 0% (control) respectively.Cell growth was tested by MTT assay and the cell relative growth rate (RGR) was calculated.The cytotoxicity was graded by generally accepted standard.Results: RGR of both cell lines cultured in nano-hydroxyapatite extraction was higher than that in control HA. In 7-day culture the cytotoxicity grade of HA was 1 in 100% of HA extraction medium and 0 in 100% of nano-hydroxyapatite extraction,or in 50% and 10% of nano-hydroxyapatite or control HA extraction medium.Primarily cultured PDLF showed lower RGR than L-929 under the same condition. Conclusion: Nano-HA do not have cytotoxic effect.
