Effect of mineralization condition medium on biological charcacteristics of bone marrow stromal cells
- VernacularTitle:矿化液对骨髓基质细胞的生物学特性的影响
- Author:
Dawei HE
;
Yan JIN
;
Xin NIE
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Bone marrow stromal cell;
Induction
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To find out the effect of mineralization condition medium on bone marrow stromal cells(MSCs) proliferation,differentiation and the secretion of extracellular matrix. Methods: MSCs of the third passage were induced by minerlization condition medium (?=10%FBS,10 nmol/L dexamethasone,50 mg/L L-vitamin C and 10 mmol/L ?-sodium glycerophosphate in DMEM). The control cells were cultured in DMEM with ?=10% FBS. The cells were observed by inverted microscope. The proliferation status and alkaline phosphatase level of cells were investigated with MTT assay. Mineralization potential was studied by Von-Kossa staining. TypeⅠ,type Ⅲ collagen and BMP synthesis were examined by immunohistochemistry stains. Results:The proliferation of MSCs in mineralization condition medium was decreased, but ALP level was increased(P
