Construction of a vector expressing SBR gene
- VernacularTitle:变形链球菌SBR基因表达载体的构建和表达
- Author:
Miao ZHANG
;
Guangshui JIANG
;
Pishan YANG
- Publication Type:Journal Article
- Keywords:
Saliva binding region;
Induced expression;
Recombinant antigen vaccine;
Dental caries
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To obtain a prokaryotic expression vector containing saliva binding region (SBR) gene of Streptococcus mutans. Methods: By directional cloning method, SBR gene fragment was cloned into the expression vector pcMVT7, the recombinant plasmid pcMVT7-SBR was transformed to E.coli JM109 (DE3). The gene expression was induced with IPTG. Restriction endonuclease and DNA sequencing techniques were used to identify the recombinant plasmid DNA, and finally target protein was purified by affinity chromatography. Results:The DNA sequence of SBR in the reconstructed vector pcMVT7-SBR was in corresponding with the initial design. The C-terminal 6?His tagged SBR fusion protein was expressed in JM109(DE3) and was purified by affinity chromatography. The expression rate of target protein was 29.73%. Conclusion:The recombinant expression plasmid pcMVT7-SBR was constructed.
