PAGE-AgNO_3 staining displays AP-PCR fingerprint of mutans streptococci
- VernacularTitle:PAGE银染法显示变形链球菌AP-PCR指纹图的可行性研究
- Author:
Song LI
;
Tianjia LIU
;
Heng ZHUANG
- Publication Type:Journal Article
- Keywords:
Polyacrylamide gel electrophoresis;
Mutans streptococci;
Polymerase chain reaction
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether the AP-PCR fingerprint of mutans streptococci(MS) can be displayed by PAGE-AgNO 3 staining. Methods: Amplification products of 200 MS clinical isolates by AP-PCR was separated and stained by ?=3.5%PAGE-AgNO 3 and agarose-EB respectively. Results were compared and the agreement value of Kappa between two methods was calculated. Results: ?=3.5%PAGE-AgNO 3 discerned both homogeneity and heterogeneity of MS genotypes, just as agarose-EB,Kappa value for agreement was 1.00 . Moreover, more bands was showed by PAGE-AgNO 3 staining than by agarose-EB, so PAGE-AgNO 3 gave a clearer pattern than agarose-EB. Conclusion: AP-PCR fingerprint of MS can be displayed by ?=3.5%PAGE-AgNO 3 staining.
