DNA Damages in Peripheral Blood Lymphocytes Induced by Organic Extracts From Ambient Air Particulates With Various Diameters
- VernacularTitle:不同粒径大气颗粒有机提取物致人外周血淋巴细胞的DNA损伤作用
- Author:
Jianghong ZHANG
;
Wenmin YANG
;
Yanwu XU
- Publication Type:Journal Article
- Keywords:
Air pollutants;
DNA damage;
Single cell gel electrophoresis
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the health effects of organic extracts from ambient air particulates with various diameters on the DNA damage of human peripheral blood lymphoctys. Methods Using a 5-stage air sampler, the ambient air particulats samples were collected in a residential area of Taiyuan city and were extracted with dichloromethane, acetone and methanol. The damage of DNA in human peripheral blood lymphocytes induced by organic extracts from ambient air particulates with various diameters at doses of 25, 50, 100 ,200 ?g/ml were detected by sigle cell gelelectrophoresis assay. Results The toxic efficiency of organic extraction of ambient air particulates increased with the decreases of the diameters of particulates. The tail lengthes of the comets induced by organic extracts from ambient air particulcctes with various diameters
