Induction of a CTL response to hepatitis B viral nucleic acid vaccine in BALB/C mice
- VernacularTitle:乙肝核酸疫苗诱导BALB/C小鼠的CTL免疫应答
- Author:
Dan LIU
;
Jun SHENG
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
HBsAg;
Nucleic acid vaccine;
CTL response
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss CTL response to the hepatitis B viral nucleic acid vaccine in mice.Methods:The recombinant plasmid containing HBV S gene was directly injected intramusculary into BALB/C mice.HBsAb in sera was checked with ELISA.The recombinant plasmid was transfected P815 cells by liposome and screened by G418.Splenocytes were isolated for investigating specific CTL response. Results:HBsAb in sera of mice was tested after pcDHBs injection.HBsAg was detected in culture medium of the transfected P815 cells. Compared mice injected with pcDNA3.1,the specific CTL cytotoxity activity of mice immunized with pcDHBs was significantly enhanced.Conclusion:HBV nucleic acid vaccine could stimulate humoral immunity and CTL response of the mice.
