Dynamic changes of rat donor mycardial cell apoptosis during hypothermic preserv ation and the protective effect of melatonin
- VernacularTitle:大鼠供心保存期间心肌细胞凋亡的动态变化及褪黑素的保护作用
- Author:
Sihai GAO
;
Tiecheng PAN
;
Chenyuan YANG
- Publication Type:Journal Article
- Keywords:
Organ preservation;
Heart transplantatio n;
Rats
- From:
Chinese Journal of Organ Transplantation
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the dynamic changes of rat donor myocardial cell apoptosis during hypot hermic preservation and the effect of melatonin.MethodsEighty rats were randomly divided into two groups: experimental group with the i solated rat hearts being preserved in 4?℃ St Thomas solution with melatonin ( 0.1?mmol/L ); control group with the isolated rat hearts being preserved in 4?℃ St Thomas solution only. After preservation for 4, 8, 12, 24 and 36?h, a poptotic index was evaluated by the percentage of mycardial cells using TUNEL po sitive staining.ResultsWith the hypothermic preservation time prolonging, more myocardial cell apoptosi s was found (control group, P
