Study on calcium signal transduction pathway in hippocampal neurons of mice with vascular dementia and the effects of dihydroergocriptine
- VernacularTitle:二氢麦角环肽对血管性痴呆小鼠海马钙信号转导机制的干预作用
- Author:
Peiyuan LU
;
Yu YIN
;
Weibin WANG
;
Cuiping LIANG
;
Wenbin LI
- Publication Type:Journal Article
- Keywords:
Dementia, vascular;
Ca 2+- Calmodulin dependent protein kinase;
Hippocampus
- From:
Chinese Journal of Geriatrics
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the calcium signal transduction pathway in hippocampal neurons of mice with vascular dementia (VaD) and the intervention effect of dihydroergocriptine (DHE). Methods The mice were subjected for ischemia-reperfusion repeatedly on bilateral common carotid arteries by knots to establish the VaD models. Animals with sham-operation were taken as control group. The treating group was administered with DHE after the establishment of VaD model. The behavior changes were observed through the step-down avoidance test and water maze test on the 29th and 30th days after operation. The resting 〔Ca 2+〕 i level of hippocampal neurons was evaluated by laser scanning confocal microscopy. RT-PCR technique was used to measure mRNA expression of CaM an CaMPKⅡ in hippocampal neurons. Results The resting 〔Ca 2+〕 i level in model group(43.50?3.00) was significantly higher than those in the sham-operation group (25.50?3.50) (P
