The inhibition of the malignant phenotype of human hepatic carcinoma cell line by antisense ?-catenin cDNA
- VernacularTitle:?-连环蛋白反义cDNA对肝癌细胞恶性表型的影响
- Author:
Kaiyi FAN
- Publication Type:Journal Article
- Keywords:
catenin;
antisense ribonucleotide;
hepartic carcinoma;
cultured tumor cells
- From:China Oncology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the effect of blocking ?-c at enin expression on the malignant phenotypes of human hepatic carcinoma cell line SMMC-7721 by antisense ribonucleotide technique. Methods:The recombinant plasmid containing antisense ?-catenin cDNA was constructed and tr ansfected into SMMC-7721. The malignant phenotypes of SMMC-7721 were analyzed by cell growth, potential colony formation in soft agar and cell cycle. Results:The morphology of SMMC-7721 cells with reduction of ?-catenin gene expression level showed the appearance of nontransformed epithelial cells. Their growth ra te and colony-formation ability in soft agar were inhibited . Cell cycle analys is by flow cytometry showed that increase in cell population in the G 0 -G 1 phrase was associated with an arrest of S phase. C-myc mRNA, a target g ene regulated by ?-catenin/TCF complex, was down-regulated in the AS-7721 ce lls. Conclusions:These results suggest that reducing ?-catenin can partially reverse malignant phenotypes of human hepartic carcinoma cell line.
