Expression of Epstein-Barr virus gene BARF1 in the nasopharyngeal carcinoma and its significance
- VernacularTitle:EB病毒基因BARF1在鼻咽癌细胞中的表达及意义
- Author:
Lei ZHANG
;
Xiaohua WANG
;
Xiulan MA
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus;
nasopharyngeal carcinoma;
BARF1 gene
- From:
China Oncology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the expression of Epstein-Barr virus gene BARF1 in nasopharyngeal carc inoma cells, to uncover the mechanism and effects of Epstein-Barr virus in the pathogenesis of EBV related carcinomas, and to offer some experimental supports for the prevention and treatment of EBV related carcinomas Methods :After RNA extraction from nasopharyngeal carcinoma samples, RT-PCR was con ducted to amplify the EBV gene EB virus associated nuclear antigen-1 (EBNA1) an d the new gene Bam HI A open reading frame 1 (BARF1) Then the PCR products wer e electrophoresed by 2% agarose gel and the product bands were detected and phot ographed Results:Of the 13 samples, 11 were qualified for the RNA qualit y And all of the 11 NPC cases were EBNA1 positive, showing the EB virus infect ion in the carcinoma cells 9 of the 11 cases were BARF1 positive, the positive rate was nearly 82% Conclusions:The EB virus gene BARF1 was highly expressed in the NPC samples This implies t hat apart from the latent membrane protein 1 (LMP1), BARF1 may play an important role in the pathogenesis of NPC The exact mechanisms of the BARF1 effects on the pathogenesis of NPC need to be studied further
