Effect of estrogen on the osteogenic ability of osteoblast
- VernacularTitle:雌激素对离体成骨细胞成骨能力的影响
- Author:
Jianhui YANG
;
Miao LIU
;
Ru HUANG
;
Xiaodong SHEN
- Publication Type:Journal Article
- Keywords:
auecifemine;
osteoblast;
alkaline phosphatase;
os teocalcin;
osteogenic ability
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the effects of different conc entrations of auecifemine on the function of osteoblast cell expression and oste ogenic ability in vitro . Methods Iliac spongy bone specimens were obtained from twel ve adult patients scheduled surgery. After the bone pieces were treated with col lagenase-trypsin, osteoblasts were obtained from cancellous bones. Then the ost eoblasts were purified and cultivated. They were cultured in medium with various concentrations(1?10 -11 , 1?10 -9 , 1?10 -7 , 1?10 -6 , 1 ?10 -5 , 5?10 -5 mol?L -1 ). The alkaline phosphatase (ALP) acti vity, osterocalcin and osteogenic ability were examined by biochemical assay and radioimmunologic assay (RIA), respectively. Results The co rrelation of auecifemine of different concentrations with ALP activity and osteo calcin secretion was positive. Auecifemine of lower concentrations (1?10 -11 mol?L -1 , 1?10 -9 mol?L -1 and 1?10 -7 mol?L -1 ) s howed the stimulating effect on osteogenic ability and but not higher concentrat ion. Conclusion Estrogen can increase osteoblast ALP activi ty, osteocalcin production, and osteogenic ability.
