Effect of extracellular signal-regulated protein on hepatic fibrosis autoreversibility and its mechanism
- VernacularTitle:细胞外信号调节激酶对肝纤维化逆转的影响及其机制
- Author:
Qin PAN
- Publication Type:Journal Article
- Keywords:
extracellular signal-regulated protein kinase;
liver fibrosis;
reversibility
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of extracellular signal-regulated protein kinase (ERK) on hepatic fibrosis autoreversibility and its mechanism. Methods: Animal model of hepatic fibrosis autoreversibility was established 6 weeks after 8-week CCl_(4 ) exposure. Then the activation, expression and distribution of ERK in the liver were investigated by Northern blot and immunohistochemistry during fibrosis reverse. RT-PCR and cDNA hybridization were used to detect the RNA and protein expression of ERK substrates. Results: The transcription of ERK was highly activated throughout the hepatic fibrosis (recovery.) ERK was located in the nucleus of hepatic stellate cells (HSCs) in fibrotic liver while it was mainly expressed in the hepatocyte cytoplasm of the lobule, sinusoid space, and perifibrous tissue as hepatic fibrosis resolved. Downstream molecules of ERK like S6 protein kinase (RSK1), c-fos, phospholipase A2, ion channels, and gastrointestinal hormone receptors were also found up-regulated during the whole course. Conclusion: ERK may be closely related to hepatic fibrosis reversibility and it may protect and promote proliferation of hepatocyte and induce apoptosis of HSC.
