Study on invasion-related factors of three human urinary bladder cancer cell lines
- VernacularTitle:三个膀胱癌细胞系侵袭相关分子的研究
- Author:
Wei CHEN
;
Xu LI
;
Xiaoli CHENG
;
Rong LIN
- Publication Type:Journal Article
- Keywords:
urinary bladder cancer;
cell line;
invasion
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the various mechanisms o f invasion in urinary bladder cancer. Methods The cloni ng efficiency and activity of adhesion of basement membrane were analysed. The f luidity of the membrane and the expression of E-cadherin, and MMP-2, and CD44 were carried out by fluorescence polarization, immunohistochemical staining and flow cytometry. Results The cloning efficiency of BLX and B LZ was 6.0% and 3.0% in soft agar, respectively. The colony of BLS was n ot formed in soft agar. The fluidity of the membrane was different in the three cell lines. The expressions of E-cadherin and CD44 in BLS and BLZ were greater than that in BLX cell line. Significant difference in MMP-2 was not found in th e three cell lines. Conclusion The research suggests that d ifference in fluidity of the membrane and expression of CD44 may play an importa nt role in invasion of urinary bladder cancer.
