Action of blocking renin-angiotensin system on protein kinase C?Ⅱ isoform in renal tissue of diabetic rats
- VernacularTitle:阻断肾素-血管紧张素系统对糖尿病大鼠肾脏蛋白激酶C?Ⅱ亚型的影响
- Author:
Li ZHANG
;
Ji MA
;
Yong GU
;
Shanyan LIN
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Protein kinase C;
Isoenzymes
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and translocation of protein kinase C (PKC) ?Ⅱ isoform in renal tissue of diabetic rats and the effects of blocking renin-angiotensin system (RAS) on them. Methods Diabetic rats induced with streptozotocin were randomized to 4 groups. (1) Diabetic control without treatment. (2) Treatment with irbesartan (40 mg?kg -1 ?d -1 ). (3)Treatment with fosinopril (40 mg?kg -1 ?d -1 ) and (4) Treatment with a combination of irbesartan and fosinopril (20 mg?kg -1 ?d -1 each). Six normoglycemic rats served as normal control. After 4 weeks, blood glucose and insulin were measured and expression and translocation of PKC?Ⅱ in renal cortex and medulla were assessed by immunohistochemistry and Western blot. Results The expressions of total and membrane fraction in renal cortex of diabetic control rats weredecreasedto66.0%and50.0% of the values of normal control rats respectively (both P
