Molecular cloning and characterization of new SLA DQA alleles and their significance
- VernacularTitle:中国猪种DQA新等位基因的克隆和分析
- Author:
Fuxiang CHEN
;
Jin XIE
;
Yong ZHANG
;
Baihua SHEN
;
Guangyan ZHOU
- Publication Type:Journal Article
- Keywords:
Swine leukocyte antigen (SLA) DQA gene;
Major hisocompatibility complex;
Xenotransplantation
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To lay background for studying rejection mechanisms in xenotransplantation, class II DQA genes of swine leukocyte antigens (SLA) of three Chinese pig strains GXP, GZP and YNP were cloned and sequenced.Methods:RT-PCR was performed to proliferate SLA-DQA genes (GXPDQA, GZPDQA and YNPDQA),which were cloned into pGEM-T Easy vector for sequencing and analysis.Results:All three allelic sequences examined, are not identical to those reported, which allows the sequences receiving their accession numbers from GenBank as AY102473, AY102474 and AY102475, which encompass an open reading frame of 765, 768 and 768 nucleotides respectively.Conclusion:Three new alleles of SLA-DQA genes were obtained. Analysis of the amino acid sequences of swine, human and mouse DQA (or equivalent) also indicated that GXPDQA, GZPDQA and YNPDQA were more similar to human DQA than mouse H-2-A?, which might provide an understanding of how the immune system evolved.
