A lungs-specific liposomal delivery system for gene therapy
- VernacularTitle:一种适用于肺脏转基因研究的脂质体运载系统
- Author:
Jianhui LIANG
;
Jun XU
;
Nanshan ZHONG
- Publication Type:Journal Article
- Keywords:
Liposome;
Gene therapy;
Targeting;
GFP
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct and evaluate a Salmeterol lungs-specific liposomal delivery system.Methods:①Liposome were prepared by the reverse-phase evaporation method and instilled into the tracheas of SD strain rats.The influence of Salmeterol on the uptakes of liposome was evaluated.②pEGFPC1(25 ?g/rodent) encapsulated in lungs-specific liposome were administered intravenously into Guinea pigs,GFP expression were observed by means of fluorescent microscopy 24h and 48h after administration.Results:①2,4,8,12,16 hours after instillation,lungs-specific liposome uptake were significantly higher than nonspecific liposome uptake(P
