Effect of 8-Br-cAMP and IPP on the expression of tau protein in various neural cell lines
- VernacularTitle:8-溴-环磷酸腺苷和异戊烯焦磷酸不改变神经细胞系tau蛋白的表达
- Author:
Jie ZHENG
- Publication Type:Journal Article
- Keywords:
Cyclic AMP;
Phosphoribosyl pyrophosphate;
Tau proteins;
Nerve cells
- From:
Chinese Journal of Geriatrics
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of 8-Br-cAMP and isopentenyl pyrophosphate (IPP) on the ex pression tau protein in various neural cell lines at the level of signal transdu ction. MethodsNG108-15, PC12 and SH-SY5Y cells were treated with 8-Br-cAMP and IPP in the 37?C incubator for 30 min, then the tau-enriched samples were analyzed by West ern blot. ResultsSH-SY5Y cells only expressed the isoform of tau protein with the lowest molecul ar weight; PC12 cells mainly expressed high molecular weight tau, and several ot her isoforms of tau; NG108-15 expressed a little of isoform of tau. In the expe rimental conditions, the expression of tau was altered with 8-Br-cAMP and IPP in NG108-15, SH-SY5Y and PC12 cells. Conclusions8-Br-cAMP and IPP does not alter the expression of tau in NG108-15, SH-SY5Y and PC12 cells.
