Adenoviral-mediated expression of human tumor suppressor gene PTEN inhibits proliferation and invasive ability of human prostate cancer cell lines LNCaP and DU-145
- VernacularTitle:人抑癌基因PTEN对前列腺癌细胞系生物学行为的影响
- Author:
Yinghao SUN
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Genes
- From:
Chinese Journal of Urology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of exog en ous adenovirus-mediated gene transfer of human tumor suppressor gene PTEN (phos phatase and tensin homology deleted on chromosome 10) on cell prolifiration and invasive ability of human prostate cancer cell lines LNCaP and DU-145. Methods mRNA and protein expressions of PTEN in human prostate c ancer LNCaP and DU-145 cells infected with AdPTEN were detected by RT-PCR and Western blot.MTT assay was used to assess the effect of PTEN on cell proliferati on.The change of cell cycle and apoptosis were measured by flow cytometry and th e invasion abilities in vitro detected by Boyden chamber.Ad-X-TRE-?gal v irus was used as control. Results RT-PCR and Western bl ot analysis showed that LNCaP and DU-145 cells express high level of PTEN after its infected with Ad-PTEN. The growth of LNCaP and DU-145 cells treated with Ad-PTEN was significantly more inhibited than that with a control adenovirus.Ad -PTEN-infected LNCaP and DU-145 cells showed a significant increase in G 0～ G 1 phase and PTEN-induced apoptosis in comparison with the control.The invasiv e ability of prostate cancer cell lines was inhibited by expression of PTEN effe ctively. Conclusions Adenoviral-mediated expression of human tumor suppressor gene PTEN has inhibitory affect on proliferation and inva sive ability of human prostate cancer cell lines LNCaP and DU-145.This study pr ovides an idea for the further development of Ad-PTEN as a potential therapeuti c vector in the gene therapy of prostate cancer.
