Histological and biochemical comparison of residual ear cartilage between microtia patient and normal counterpart
- VernacularTitle:小耳畸形残耳软骨的生物化学研究
- Author:
Ji JIN
;
Hongxing ZHUANG
- Publication Type:Journal Article
- Keywords:
Microtia;
Ear cartilage;
Biochemistry
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2002;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biochemical differences between the cartilage of the residual ear of microtia patient and normal person. Methods Seven cartilage specimens from 7 about 10-years-old microtia patient's residual ears and 7 normal ears from cadaver of the same age were measured by electrophoretic mobility shift assay and spectrophotometer for collagen glycoaminoglycan (GAG), chondroitin sulfate (Chs), hyaluronan (HA) and keratin sulfate (KS) .Results Biochemical analysis showed that no significant difference were found in the collagen contents of the two groups. The microtia cartilage had more GAG (49.00?25.60) ?g/mg than that of the normal (28.25?4.80) ?g/mg. The composition of GAG were different between two groups: microtia group (HA 38.96+4.97 %, Chs 29.02 %?4.12 %, KS 32.16 %?7.41) % and normal group (HA 32.94 %?3.24 %, Chs 33.10 %?2.61%, KS 33.96 %?1.66 %). There were differences in HA and Chs relative contents, but not in KS between the two groups. Conclusions There is no difference in the collagen content between normal and microtia ear cartilage. But the latter has more GAG than the former. As for the constitution of GAG, microtia cartilage has a higher percentage of HA, lower percentage of Chs, and no significant difference in KS.
