Effects of TGF-?_1 on activity of protein tyrosine kinase in scar tissue of rabbit ear
- VernacularTitle:?_1转化生长因子刺激兔耳皮肤瘢痕增生中蛋白酪氨酸激酶活性的变化
- Author:
Dalie LIU
;
Xuanfen ZHANG
;
Kai LIU
- Publication Type:Journal Article
- Keywords:
Signal transduction;
Scar;
Protein tyrosine kinase;
TGF-?_1
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2002;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of protein tyrosine kinase (PTK) activity in scar tissue of rabbit ear induced by TGF-?_1 and to investigate the signal mechanism of TGF-?_1 in the scar proliferation. Methods TGF-?_1 (5 ng/ml) was applied on the wound and in the scar of rabbit ear. The PTK activity in the tissues from normal control, and 0, 14, 30, 45, and 60 d after wound epithelization was measured by phosphorus ( 32 P) incoporation. The scar changes were also observed. Results PTK activity in hypertrophic scar tissue [from (4.40?1.31) to (4.91?1.32)pmol?min -1 ?mg -1 ] was obviously elevated as compared with(2.87?0.96) pmol?min -1 mg -1 of normal skin ( P 0.05). Furthermore, PTK activity of hypertrophic scar tissue was obviously elevated as compared with that of normal scar tissue ( P 0.05) but could accelerate scar hypertrophy ( P
