Construction of specific ribozymes against second exon of ?_1 Ⅰ and ?_1 Ⅲ procollagen genes
- VernacularTitle:针对?_1 Ⅰ型及?_1 Ⅲ型前胶原基因第二外显子片段核酶的构建
- Author:
Jiayuan ZHU
;
Bin ZHU
;
Bing XU
- Publication Type:Journal Article
- Keywords:
Procollagen gene;
Ribozyme;
Consturction
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2002;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct two ribozyme genes (gⅠand gⅢ) by using PCR according to the requirement of ribozyme's domains and the nucleotide sequences of ?_1Ⅰand Ⅲ procollagen genes. Methods First we constructed two ribozyme genes (g, gⅢ) by using PCR, and then separately cloned into the downstream of T7 promoter of pGEM-T vector. Results The resulting recombinant plasmids (pT-gⅠ, pT-gⅢ) were verified by restriction enzymes, PCR and sequencing. Conclusion The expected two ribozyme genes are constructed successfully and this work affords the basis for the preparation of large amounts of ribozyme molecules and for the study on interaction between mRNA and ribozyme in vivo.
