Allogeneic transplantation of myoblasts seeded on typeⅠcollagen sponges in rats
- VernacularTitle:组织工程化成肌细胞植入同种动物体内的研究
- Author:
Guilin HUANG
;
Longjiang LI
;
Xueying LI
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Myoblasts;
Histology
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
s Objective: To study the feasibility of allogeneic tra ns plantation of myoblasts of SD rats for the reconstruction of facial muscle de fects. Methods: Myoblasts obtained from the forelimbs and hindlimbs of neonatal SD rats were purified and cultured. 5?10 4 myoblasts w ere seeded onto each of type Ⅰcollagen sponges in the size of 1.5 mm?0.8 mm? 0.5 cm. The myoblasts/sponge constructs were transplanted into the facial muscl e defects of syngeneic mutured rats and observed with microscopy,immunohistoche mistry and electromyography (EMG).Result:Myoblasts attac hed and kept alive on type I collagen for at lest 3～4 days in culture. 4 and 8 w eeks after transplantation,muscle like tissue with blood vessles was observed i n the implants. Expression of actin and myosin in the implants was similar to th at in the control muscle.8 weeks after implantation,the spike wave (mV) on the g rafted side and control side was 0.7?0.3 and 2.7?0.6 ( P
