Rapid genotyping for HLA-DR52-associated group by oligoneucleotide arrays
- VernacularTitle:基因芯片快速HLA-DR52组分型
- Author:
Jiaquan XIAO
;
Jianming TAN
;
Chengtao LI
- Publication Type:Journal Article
- Keywords:
Genes, MHC class Ⅱ;
DNA probes, HLA;
Genotype;
Oligoneucleotide array
- From:
Chinese Journal of Organ Transplantation
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an oligoneucleotide array for HLA-DR52 group rapid genotyping.Methods According to the special allele sequences of HLA-DRB loci in Chinese Han's population, HLA-DR52 group typing probes which were immobilized on a glass supports were synthesized. A pair of group-special primers labeled by the Cy5-dCTP were designed and were used in the PCR. The labeled PCR products with Cy5 were hybridized with array. The signals were scanned by a scanner and analyzed by Image software. 83 samples were typed by this array and the results were compared with PCR-SSP typing.Results Among 57 HLA-DR52 group loci typed by PCR-SSP,2 samples had no HLA-DR52 loci typed by array,3 DR52 group homozygotes typed by PCR-SSP were actually heterozygotes by array. The other 1 non-DR52 group homozygote identified by PCR-SSP was a heterozygote with one DR52 group locus. Conclusion The oligoneucleotide array technique is a precise, rapid molecular method for HLA-DR52 genotyping. Compared with PCR-SSP method, the genotyping chip is more sensitive and intuitionistic and suitable for clinic practice.
