Combined use of vitamin D-derived drug and DBMC to induce tolerance on heterotopic rat cardiac allograft
- VernacularTitle:维生素D衍生物联合供者骨髓细胞输注诱导异基因大鼠心脏移植免疫耐受
- Author:
Jianghua CHEN
;
Rui JIANG
;
Qiang HE
- Publication Type:Journal Article
- Keywords:
Calcitriol;
Heart transplantation;
Bone marrow cells;
Tissue donors;
Immune tolerance
- From:
Chinese Journal of Organ Transplantation
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of 1,25-(OH) 2-D 3 in combined with DBMC to induce tolerance on heterotopic rat cardiac allografts.Methods After the establishment of a stable rat cervical heterotopic heart transplantation model, inbred male Wistar rat hearts were transplanted to male SD rats. The experimental rats were divided into the following groups: Group A served as experimental control group, group B receiving 1,25-(OH) 2-D 3 5000 ng/kg, group C being treated with 1?10 8 DBMC, group D being treated with 1?10 8 DBMC in combined with 1,25-(OH) 2-D 3 5000 ng/kg and group E being treated with CsA 10?mg/kg. The survival time of donor hearts was observed, meanwhile, the donor specific mixed lymphocyte reaction (MLR) and concentrations of TNF-alpha and ICAM-1 in grafts were measured. HE staining inspected all specimens of heart grafts.Results The mean graft survival time (days) in the groups A, B, C, D and E group was 7.2 ? 1.6 , 10.6 ? 2.3 , 12.4 ? 2.5 , 68.3 ? 9.9 and 17.5 ? 4.4 , respectively. The results showed there were significant differences in graft survival time between the group D and the other groups ( P
