Investigation of the cold ischemia preservation of human pancreases islets
- VernacularTitle:成人胰岛细胞的分离
- Author:
Hongjun GAO
;
Minzhuan LIN
;
Min LI
- Publication Type:Journal Article
- Keywords:
Islets of Langerhans;
Cell separation;
Ischemia;
Cells;
Survival rate
- From:
Chinese Journal of Organ Transplantation
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new method of cutting human pancreases from cadaver donors for islets transplantation, and to discuss the relationship between activity of islets and the cold ischemia preservation time after hypertonic citrate adenine solution perfused.Methods 1500-2000 ml of hypertonic citrate adenine solution was perfused into aorta. Kidey-pancreases-duodenum-spleen were cut together. Digestion of pancreases was done with collagenase P. The islets were isolated, purified and stained. The purity and activity of the islets were measured.Results There was a negative correlation between activity of islets and the cold ischemia preservation time. But there was a positive correlation between activity of islets and the ischemic index.Conclusion The activity of the islets infused with hypertonic citrate adenine solution and degested with collagenase P is high, but the cold ischemia preservation time of the pancreases preserved in hypertonic citrate adenine solution is better not more than 5-6 h.
