Analysis of acute arsenite-induced L-02 cells by cDNA microarray
- VernacularTitle:急性砷染毒的L-02细胞的基因芯片分析
- Author:
Yongqing GU
;
Lei YANG
;
Guoquan WANG
;
Bingxiang YUAN
;
Zemin PAN
;
Kang YING
;
Yao LI
;
Yi XIE
- Publication Type:Journal Article
- Keywords:
L-02 cell;
cDNA microarray hybridization;
sodium arsenite
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression profile of human genes in response to acute sodium arsenite treatment by cDNA microarray. Methods The RNA was purified from the L-02 cells without and with arsenite sodium induction for 2 hours, 15 hours and 24 hours, respectively. Results The hybridization patterns were different between every interval of arsenite induction. Expression of hCYR61 increased after 2 hours' induction, but decreased after 15 hours and 24 hours. Expression of metallothionein Ⅳ and Ⅲ elevated at the whole induction phase. HSP86 was up-regulated after 15 hours and 24 hours' induction, but it did not alter at two hours' induction. Conclusion When exposed to arsenite, the cells are under a meet-an-emergency situation to synthesize the most necessary protein and inhibit synthesis of unessential proteins.
