Preparation of nifedipine liposomes by pH steps degree-freeze thawing method
- VernacularTitle:pH梯度-冻融法制备硝苯地平脂质体
- Author:
Weifeng LI
;
Xiaofeng NIU
;
Haiying CHEN
;
Guilan ZHAO
- Publication Type:Journal Article
- Keywords:
nifedipine;
liposome;
stability
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preparation of nifedipine liposomes (NP) and evaluate their quality. Methods The liposomes were prepared by a pH steps degree-freeze thawing method, with the entrapment and stability as indices. The entrapment was studied by Sephadex G-50 minicolumn centrifugation UV-Vis spectroscopy method. Entrapment, and degree distribution before and after freezing were compared, the effect of freezing times on entrapment was studied. Results The entrapment and the average size of NP liposomes were 96.65% and 2.0 ?m, respectively. After freezing, both the entrapment and stability were increased. Conclusion The selected formulation and preparation of nifedipine liposomes are practicable.
