Expression of cyclooxygenase-2 and inducible nitric oxide synthase in cervical carcinomas and its significance
- VernacularTitle:环氧合酶-2和诱导型一氧化氮合酶在宫颈癌组织中的表达及其意义
- Author:
Wei LI
;
Rujun XU
;
Honghe ZHANG
- Publication Type:Journal Article
- Keywords:
cervical carcinomas;
cyclooxygenase-2;
nitric oxide synthase
- From:China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate the relation of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) to tumor progression of cervical carcinoma. Methods:The expression and localization of COX-2 and iNOS protein in the 25 patients with cervical carcinomas were determined by immunohistochemical and the gene expression of COX-2 and iNOS were examined by reverse-transcription polymerase chain reaction ( RT-PCR). Results:Immunohistochemical staining for COX-2 and iNOS expression was strongly positive in 15 of 25 (60 %) and 20 of 25 (80 %) cases,respectively. Increased COX-2 and iNOS mRNA levels were confirmed by RT-PCR. There was negative correlation between COX-2 expression and tumor cell differentiation(r=-0.420, P
