Expression of MEK-1 in regulation of ?V integrin and E-cadherin of MCF-7 breast cancer cells
- VernacularTitle:MEK-1对MCF-7乳腺癌细胞?V整合素和上皮钙粘素调节的表达
- Author:
Yong CAI
;
Lianfeng ZHANG
- Publication Type:Journal Article
- Keywords:
MEK-1;
?V integrin;
E-cadherin;
cultured tumor cells
- From:
China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:Expression of MEK-1 was studied by regulating ?V integrin and E-cadherin on MCF-7 breast cancer cells.Methods:Using cell transfected techniques site-mutated genes were inserted into MCF-7 cells and made MCF-7 cells containing MEK-1-GFP,Ala 222 MEK-1-GFP,Asp 222 MEK-1-GFP ,respectively. These cells were tested with immunoblot analysis and immunofluoresence technique to determined the change of MEK-1 expression and location of MEK-1 in cells.Results:Deactivated Ala 222 MEK-1-GFP inhibited the activity of MAPK,active Asp 222 MEK-1-GFP increased the activity of MAPK. Ala 222 MEK-1-GFP increased the expression of E-cadherin,did not increase the expression of ?V integrin and inhibited FAK phosphorylation. MEK-1-GFP and Asp 222 MEK-1-GFP did not affect the expression of E-cadherin ,but upregulated the expression of ?V integrin,increased FAK phosphorylation.Conclusions:MEK-1 regulated the expression of ?V integrin and E-cadherin,FAK is the key factor that induced signal pathway of ?V integrin. It is possible that MEK-1 regulated FAK phosphorglation by regulating expression of ?V integrin and E-cadherin.
