Reversal effect on ADM resistance in human leukaemia cell subline resistant to ADM(K562/ADM) of anti-FasL
- VernacularTitle:FasL抗体对人白血病细胞株(K562/ADM)多柔比星耐药性的逆转作用
- Author:
Tianrong ZHENG
;
Dongmei ZHOU
;
Zuofu XIE
- Publication Type:Journal Article
- Keywords:
anti-FasL;
apoptosis;
multidrug resistance;
reversion of drug resistance;
leukaemia cell line
- From:
China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To explore the effect of anti-FasL on ADM resistance of K562/ADM cell subline and its possible mechanism.Methods:Control group,untreated with VER or anti-FasL ,VER-treated group,anti-FasL-treated group and anti-FasL +VER-treated group were set up. MTT、FCM、TUNEL、S-P immunechemistry and RT-PCR assay were used for detection.Results:Anti-FasL or VER treatment was capable of strengthening sensitivity of K562/ADM cells to ADM,ID 50 of anti-FasL and VER was 200ng/ml and 6?g/ml,respectively;DNA content of apoptosis from cycling analysis curve of K562/ADM cell was 7.42%,32.10%,49.29%,56.26%,respectively,and positive rate in situ apoptosis was 4.5%,36%,45%,58%,respectively,which represented a tendency of gradual increase. Anti-FasL was capable of effecting FasL and bcl-2 expression,but not Pgp.Conclusions:Anti-FasL alone or synergically with VER was capable of reversing ADM resistance of K562/ADM cell,which may be used as a novel reversor of multidrug resistance.
