Relation between paraoxonase 1 gene Q191R polymorphism and type 2 diabetes mellitus complicated with hypertension
- VernacularTitle:对氧磷酯酶1基因Q191R多态性与2型糖尿病合并高血压的关系
- Author:
Yunling HAO
;
Lixiang LIN
;
Gang CHEN
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus, non-insulin-dependent;
Hypertension;
Paraoxonase;
Gene;
Polymorphism (Genetics)
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relation between paraoxonase (PON) 1 Q191R gene polymorphism and type 2 diabetes mellitus complicated with hypertension. Methods Genomic DNA was extracted from peripheral blood leukocytes. The studied DNA was amplified by PCR, and then digested with the restriction endonuclease AlwⅠ and separated on 2% agarose gel electrophoresis. PON activity was estimated by spectrophotometer. Serum insulin and C-peptide were measured by radioimmunoassay. Results The frequencies of QQ genotype and Q allele in type 2 diabetes mellitus with hypertension group were significantly higher than those in type 2 diabetes mellitus group (P
