Effects of insulin on nitric oxide synthase (NOS) activity and the expression of inducible NOS mRNA in calf aortic endothelial cells
- VernacularTitle:胰岛素对牛主动脉内皮细胞一氧化氮合酶(NOS)活性及诱生型NOS mRNA表达的影响
- Author:
Hui ZHANG
;
Xiangfang CHEN
;
Fulin YE
;
Zhimin LIU
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Endothelial cells;
Nitric-oxidase synthase
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe nitric oxide synthase (NOS) activity and the expresssion of inducible NOS (iNOS) mRNA in cultured calf aortic endothelial cells treated with various concentrations of insulin. Methods Cultured calf aortic endothelial cells were incubated with different concentrations of insulin for 24 h. NOS activity was determined by colorimetry and iNOS mRNA was measured by semi-quantitative RT-PCR. Results NOS activity and the expression of mRNA in calf aortic endothelial cells at pharmacological concentration (10 -7 mol/L) of insulin were significantly increased than those at physiological concentration (10 -10 mol/L) of insulin (P
