Effect of 1,25-dihydroxyvitamin D_3 on immune intervention in NOD mice
- VernacularTitle:1,25-(OH)_2维生素D_3对NOD小鼠的免疫干预作用
- Author:
Nianhuan CHANG
;
Lina WANG
;
Zhili ZHANG
- Publication Type:Journal Article
- Keywords:
Calcitriol;
? cell;
Apoptosis;
Diabetes mellitus, insulin-dependent;
Cyclophosphamide
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preventive effect of 1,25-dihydroxyvitamin D_3 〔1,25-(OH)_2D_3〕 on type 1 diabetes in cyclophosphamide-treated NOD mice. Methods Twenty female NOD mice were administered 280 mg/kg cyclophosphamide by a single intraperitoneal injection at the beginning of the experiment and then divided into two equal groups. Group 1 received intraperitoneal injection of 5 ?g/kg 1,25-(OH)_2D_3 every other day; Group 2 received the intraperitoneal injection of peanut oil in the same volume as control. When the experiment was finished on the 30th day, the incidence of diabetes and the degree of insulitis were observed. The expressions of bcl-2 and bax in islets were detected with immunohistochemical technique. The apoptosis rate of spleen T lymphocyte was also measured by flow cytometry quantitative analysis. Results Intraperitoneal injections of 1,25-(OH)_2D_3 to NOD mice reduced the incidence of diabetes (10% vs 70%, P
