The killing activity of IL-6+CD3AK cells against the MGC-803 gastric carcinoma cell in vitro
- VernacularTitle:IL-6+CD3AK细胞对MGC-803胃癌细胞株杀伤作用的体外实验研究
- Author:
Taiqin HUANG
;
Jingzi JIN
;
Su SUN
- Publication Type:Journal Article
- Keywords:
Tumor killer;
Gastric carcinoma;
Adoptive immunotherapy
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The killing activity of tumor killer cells against the MGC-803 gastric carcinoma cells was investigated Methods:The IL-6+CD3AK cells were prepared from the peripheral blood mononuclear cells (PBMCs) from the culture media containing rIL-6 with rIL-2 and anti-CD3Ab The other control groups were cultivated in rIL-6 only, in rIL-6 with anti-CD3ab, in rIL-2 only Each group was divided into four groups by the ratio of effect and target cells After 24 h and 48 h, the death rate of cancer cells was determined by MTT assay and trypan-blue exclusion, the morphologic changes were observed by various microscope Results:The killing activity of rIL-6+CD3AK cells was higher than those of the other groups(P
