The biologic character and inducing to the HBV-specific cytotoxic T lymphocytes of DCs from the patients with chronic hepatitis B pulsed with HBsAg in vitro
- VernacularTitle:HBsAg体外冲击的慢性乙肝患者树突状细胞的生物学特性及其对HBV特异性CTL的诱导作用
- Author:
Xiaoying WANG
;
Xiaolei SUN
;
Wei TANG
- Publication Type:Journal Article
- Keywords:
Chronic hepatitis B;
Dendritic cell;
HBsAg;
Cytotoxic T lymphocyte (CTL)
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the functional status of the monocytes-derived dendritic cells (DCs) plused with HBsAg from patients with chronic hepatitis B and their capacity of inducing the HBV- special cytotoxic T lymphocytes (CTLs),and explore a new method of inducing the special anti-HBV cell-mediated immunity.Methods:Monocytes of patients were isolated from peripheral blood and incubated with GM-CSF+IL-4+TNF-? to induce DCs generation. Those DCs were pulsed with HBsAg to induce HBV- special DCs. The phenotype of DCs including CD1a,CD80,CD83,CD86,CD40 and HLA-DR was characterized by FCM and the stimulating reaction of allogenic T lymphocytes was detected by MTT assay. The concentration of cytokines such as IL-12 and IL-6 in supernate was tested by ELISA and the cytotoxicity of CTLs inducing by the DCs against HepG2 2.2.15 cells,HepG2 cells and K562 cells were detected by LDH assay.Results:The expression of CD1a, CD80, CD83,CD86, CD40, HLA-DR molecules on the DCs pulsed with HBsAg was higher than those of the control group (P
