Immunofluorescence studies on the skin sections of recessive dystrophic epidermolysis bullosa patients with anti-P_ (200) pemphigoid sera
- VernacularTitle:隐性营养不良性大疱性表皮松解症皮肤的免疫荧光研究
- Author:
Yuanlin LIU
- Publication Type:Journal Article
- Keywords:
Anti-P 200 pemphigoid;
Immunoblotting;
Immunofluorescene;
Recessive dystrophic epidermolysis bullosa;
Type Ⅶ collagen
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to determine the nature of P 200 autoantigen Methods:12 cases of anti-P 200 pemphigoid sera were collected The skin sections from 6 cases of recessive dystrophic epidermolysis bullosa were studied with these sera by indirect immunofluorescence Results:All the 12 anti-P 200 pemphigoid sera could react with basement membrane zone (BMZ)of 5 cases of recessive dystrophic epidermolysis bullosa, while epidermolysis bullosa acquisita sera were negative in these skins In addition, in a case of recessive dystrophic epidermolysis bullosa, epidermolysis bullosa acquisita sera react with both BMZ and intracytoplasmic deposition of type Ⅶ collagen, while no anti-P 200 pemphigoid sera showed this reactivity Conclusion:These results suggested that the 200 kD antigen is not a component of type Ⅶ collagen, but a specific autoantigen
