Repair of segmental radial defects with composite of human fibrinous gel and transgeneic MSCs
- VernacularTitle:转基因骨髓间充质干细胞复合纤维蛋白凝胶修复兔桡骨缺损
- Author:
Guangrong JI
;
Xin LIN
;
Jianfeng HAN
- Publication Type:Journal Article
- Keywords:
Fractures;
Mesenchymal stem cells;
Tissue engineering;
Bone morphogenetic proteins
- From:
Chinese Journal of Trauma
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the feasibility of composite of human fibrinous gel and transgeneic MSCs aiming at replicating adenoviruses-bone morphogenetic proteins-2 (Ad-BMP-2) in repair of segmental bone defects. Methods Bone defects for 10 mm were created on the bilateral radius of 14 Japanese big ear rabbits and treated with four kinds of implantations (seven radii per group), ie, composite of transgeneic MSCs and fibrinous gel (Group A), composite of MSCs and fibrinous gel (Group B),fibrinous gel alone (Group C) and nothing as control group. The defects were observed radiologically and histologically and examined by biomechanical test at the 12th week after operation. Results The defects treated in the Group A were repaired and regenerated much more new bone, bridged earlier and stronger than those in the Group B 12 weeks after operation. The defects treated in the Groups C and D could not attain osseous tissue healing. Conclusions The composite of transgeneic MSCs and fibrinous gel can be used to effectively repair segmental bone defects.
