The effect of selective estrogen receptor modulator,raloxifene on the gene expression of endothelin-1 in vascular endothelial cells and the relationship with estrogen receptor
- VernacularTitle:雷洛昔芬对血管内皮素-1基因mRNA表达的影响及与雌激素受体的关系
- Author:
Hong HE
;
Falin YANG
;
Qing ZHU
;
Yinan WU
;
Xianxi LIU
;
Shuchun WANG
- Publication Type:Journal Article
- Keywords:
Selective estrogen receptor modulator;
Endothelium, vascular;
Receptors, estrogen
- From:
Chinese Journal of Geriatrics
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of raloxifene on the mRNA expression of endothelin-1 in vascular endothelial cells and assess the role of estrogen receptor. Methods Bovine carotid endothelial cells were pretreated with 10nM raloxifene for 24 hours, then total RNA was harvested and Northern blotting was performed to investigate the effect of raloxifene on the mRNA expression of endothelin-1. Furthermore, estrogen receptor inhibitor, 100nM ICI 182 780 was used to pretreat the cells together with raloxifene and the expression of endothelin-1 was observed too. Results The mRNA expression of endothelin-1 in bovine carotid endothelial cells was inhibited significantly by pretreatment with raloxifene and this effect could be blocked by ICI182 780 (0.16?0.05 vs 0.39?0.07, P
