Screening of the interaction protein with Wnt signaling molecular-TCF4 from renal carcinoma cDNA library by yeast two hybrid
- VernacularTitle:从人肾癌酵母双杂交文库中筛选TCF4相关蛋白
- Author:
Xiongjun YE
;
Guiting LIN
;
Zhiwen ZHANG
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Wnt signaling pathway
- From:
Chinese Journal of Urology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the interaction pr otein with Wnt signaling molecular-TCF4 in renal carcinogenesis.Me thodsThe N terminal and DNA binding domain of human T cell factor 4 (TCF4ND, 1～495 aa) was used as a bait to screen the renal carcinoma cDNA libra r y by yeast two hybrid.Results51 positive colonies were fished out such as ?-catenin,TCF4,activating transcription factor 5(ATF5),and 2 unknown genes.ConclusionsIt is indicated that exten sive signaling cross-talks between Wnt and other signaling pathways might play an significant role in renal carcinogenesis.
